Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and chloroplast-gene transformation of rbcLΔ-MX3312 mt+ (MUT-4696), a T342I substitution (ACT-ATT) was created in the Rubisco large subunit. This is the intragenic-suppressor substitution that complements the V331A substitution in photosynthesis-competent revertant R40-9D (rbcL-V331A/T342I). The T342I substitution alone decreases the CO₂/O₂ specificity of Rubisco, and the x-ray crystal structure of the T342I mutant Rubisco has been solved. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.

Species:

Chlamydomonas

Locus:

rbcL

Chromosome:

Chloroplast