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MUT-4810 rbcL-L326I mt+
Catalog No: ALS-03946
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and chloroplast transformation of rbcLΔ-25B1 mt+ (MUT-4700) were used to create an L326I substitution (CTT-ATT) in the Rubisco large subunit, which causes a decrease in Rubisco holoenzyme stability. This is the original mutant strain. It was created to investigate phylogenetic differences near large-subunit residue Val-331 (see rbcL-V331A). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.