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MUT-4815 rbcL-G237S pf2 mt- (67-2AA) (pf2 progeny used for genetic analysis)
Datasheet
Catalog No: ALS-03951
Product Details
Subategory:
Mutants
Description:
This chloroplast mutant was isolated by screening a large collection of acetate-requiring mutants for lack of complementation with a known rbcL mutant. The mutant was crossed with pf2 mt- to confirm uniparental inheritance, and an mt- progeny clone was analyzed. The mutant results from a GGT to AGT rbcL mutation that causes Gly-237 to be substituted by Ser in the Rubisco large subunit. The mutant strain has a decreased level of Rubisco holoenzyme and lacks carboxylase activity. Attempts to select photosynthesis-competent revertants yielded only true revertants. This strain has been maintained with acetate medium in darkness since its recovery.
Species:
Chlamydomonas
Locus:
rbcL, PF2
Chromosome:
Chloroplast,11
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.