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MUT-4818 rbcL-Q45Ocher mt- (68-7AN) (non-pf2 progeny used for genetic analysis)
Datasheet
Catalog No: ALS-03954
Product Details
Subategory:
Mutants
Description:
The 68-7AN chloroplast mutant was recovered by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant. It results from an rbcL nonsense mutation that changes the codon for Gln-45 (CAA) to ocher (TAA) (Spreitzer 1993). It is not known whether this mutant may be suppressed by heteroplasmic informational (tRNA) suppression. This strain was recovered from a cross between the original 68-7AN mt+ and pf2 mt-, and has been maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.