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MUT-4819 rbcL-T173I mt+ (69-12Q) (original isolate)
Datasheet
Catalog No: ALS-03955
Product Details
Subategory:
Mutants
Description:
The 69-12Q chloroplast mutant was recovered by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant. It results from a T173I substitution (ACA-ATA) in the chloroplast-encoded large subunit of Rubisco, which eliminates carboxylase activity but not Rubisco holoenzyme assembly. This active-site residue is normally Ile in Rhodospirillum rubrum Rubisco, and replacing that residue with Thr also causes a decrease in carboxylase activity. Selection for photosynthesis-competent revertants of rbcL-T173I yielded only true revertants. This strain has been maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.