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MUT-4827 rbcL-S379A pf2 mt- (pf2 progeny used for genetic analysis)
Catalog No: ALS-03963
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and chloroplast co-transformation of wild-type 2137 mt+ (MUT-3269) were used to create an S379A substitution (TCA-GCA), which causes a decrease in Rubisco CO2/O2 specificity but not holoenzyme stability. This strain was recovered from a cross between the original S379A mt+ and pf2 mt-, and has been maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL, PF2
Chromosome:
Chloroplast,11
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.