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MUT-4829 rbcL-D473E mt+
Datasheet
Catalog No: ALS-03965
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696) were used to create a D473E substitution (GAC-GAA) in the Rubisco large subunit, which causes a decrease in Rubisco CO2/O2 specificity but not holoenzyme stability. The x-ray crystal structure of the mutant protein has been solved. This is the original mutant strain. It was created due to a previous study of the role of Asp-473 in catalysis. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.