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MUT-4835 rbcL-L290F/A222T mt+ (R96-8E revertant of 68-4PP)
Datasheet
Catalog No: ALS-03970
Product Details
Subategory:
Mutants
Description:
R96-8E was recovered as a photosynthesis-competent revertant after methyl-methanesulfonate mutagenesis of rbcL-L290F mt+ (68-4PP). It results from an intragenic-suppressor mutation that causes an A222T substitution (GCT-ACT) in the Rubisco large subunit, which increases the CO2/O2 specificity of the original mutant enzyme. The L290F/A222T mutant-enzyme x-ray crystal structure has been solved. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.