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MUT-4836 rbcL-L290F/V262L mt+ (R96-4C revertant of 68-4PP)
Datasheet
Catalog No: ALS-03971
Product Details
Subategory:
Mutants
Description:
R96-4C was recovered as a photosynthesis-competent revertant after methyl-methanesulfonate mutagenesis of rbcL-L290F mt+ (68-4PP). It results from an intragenic-suppressor mutation that causes a V262L substitution (GTA-TTA) in the Rubisco large subunit, which increases the CO2/O2 specificity of the original mutant enzyme. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.