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MUT-4842 rbcL-C256F/K258R mt+
Datasheet
Catalog No: ALS-03977
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and chloroplast transformation of rbcLΔ-25B1 mt+ (MUT-4700) were used to create C256F (TGT-TTC) and K258R (AAA-CGT) substitutions in the Rubisco large subunit, which cause a decrease in carboxylation catalytic efficiency. This is the original mutant strain. It was created to investigate phylogenetic differences near large-subunit residue Leu-290 (see rbcL-L290F). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.