Products
Online Inquiry
MUT-4843 rbcL-C256F/I265V mt+
Datasheet
Catalog No: ALS-03978
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and chloroplast transformation of rbcLΔ-25B1 mt+ (MUT-4700) were used to create C256F (TGT-TTC) and I265V (ATT-GTA) substitutions in the Rubisco large subunit, which cause decreases in carboxylation catalytic efficiency and CO2/O2 specificity. This is the original mutant strain. It was created to investigate phylogenetic differences near large-subunit residue Leu-290 (see rbcL-L290F). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Related Products
For Research Use Only. Not For Clinical Use.