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MUT-4847 rbcL-V221C/V235I/C256F/K258R/I265V mt+ (Penta)
Datasheet
Catalog No: ALS-03982
Product Details
Subategory:
Mutants
Description:
Using a plasmid containing the rbcL-C256F/K258R/I265V gene, directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696) were used to create V221C (GTT-TGT), V235I (GTT-ATT), C256F (TGT-TTC), K258R (AAA-CGT), and I265V (ATT-GTA) substitutions in the Rubisco large subunit. The addition of the V221C and V235I substitutions increases the CO2/O2 specificity of rbcL-C256F/K258R/I265V Rubisco. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.