Subategory:

Mutants

Description:

To create the penta/ABSO mutant, rbcL-V221C/V235I/C256F/K258R/I265V mt+ was crossed with rbcSΔ-CAL005.01.13 mt- (MUT-4693), and an mt+ acetate-requiring progeny clone was recovered. That strain was then transformed with an rbcS1-ABSO plasmid, which encodes a Rubisco small subunit containing the spinach betaA-betaB loop. Penta/ABSO Rubisco has increased CO₂/O₂ specificity and other kinetic properties similar to plant Rubisco enzymes. This is the original mutant strain. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco expression.

Species:

Chlamydomonas

Locus:

rbcL, RBCS1

Chromosome:

Chloroplast,2