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Description:

MUT-4850 rbcL-L290F/rbcS2-N54S mt+ (R116-1B revertant of 68-4PP) (non-pf2 progeny used for genetic analysis)

Catalog No: ALS-03985

Product Details

Subategory:
Mutants
Description:
R116-1B was recovered as a photosynthesis-competent revertant after methyl-methanesulfonate mutagenesis of rbcL-L290F mt+ (68-4PP). It results from a mutation in rbcS2 that causes an N54S substitution (AAC-AGC) in the Rubisco small subunit, which increases the CO2/O2 specificity of the original mutant enzyme. Gene-centromere mapping indicated that rbcS2 is 20 map units from its centromere. This strain was recovered from a cross between the original revertant R116-1B mt+ and pf2 mt-. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL, RBCS2
Chromosome:
Chloroplast,2

For Research Use Only. Not For Clinical Use.