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MUT-4852 rbcL-L290F/rbcS2-A57V mt+ (R116-10C revertant of 68-4PP) (original isolate)
Datasheet
Catalog No: ALS-03987
Product Details
Subategory:
Mutants
Description:
R116-10C was recovered as a photosynthesis-competent revertant after methyl-methanesulfonate mutagenesis of rbcL-L290F mt+ (68-4PP). It results from a mutation in rbcS2 that causes an A57V substitution (GCC-GTC) in the Rubisco small subunit, which increases the CO2/O2 specificity of the original mutant enzyme. Gene-centromere mapping indicated that rbcS2 is 20 map units from its centromere. This is the original revertant strain. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL, RBCS2
Chromosome:
Chloroplast,2
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For Research Use Only. Not For Clinical Use.