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MUT-4854 rbcL-L290F/rbcS2-C65S mt+ (R88-5A revertant of 68-4PP)
Datasheet
Catalog No: ALS-03989
Product Details
Subategory:
Mutants
Description:
R88-5A was recovered as a spontaneous photosynthesis-competent revertant of rbcL-L290F mt+ (68-4PP). It results from a transversion mutation in rbcS2 that causes a C65S substitution (TGC-TCC) in the Rubisco small subunit, which increases the CO2/O2 specificity of the original mutant enzyme. See also S88-5A. Gene-centromere mapping indicated that rbcS2 is 20 map units from its centromere. This is the original revertant strain. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL, RBCS2
Chromosome:
Chloroplast,2
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For Research Use Only. Not For Clinical Use.