Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and nuclear-gene transformation of rbcSΔ-T60-3 mt- (MUT-4690), a C65S substitution (TGC-AGC) was created in the Rubisco small subunit. This is the small-subunit intergenic-suppressor substitution that complements the large-subunit L290F substitution in photosynthesis-competent revertant R88-5A (rbcL-L290F/rbcS2-C65S). The small-subunit C65S substitution alone improves the thermal stability of wild-type Rubisco. This strain has been maintained with acetate medium in darkness since its creation.

Species:

Chlamydomonas

Locus:

RBCS2