Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and nuclear-gene transformation of rbcSΔ-T60-3 mt- (MUT-4690), a C65P substitution (TGC-CCG) was created in the Rubisco small subunit. This mutant was created to investigate rbcS suppression of an rbcL mutation. See also R88-5A (rbcL-L290F/rbcS2-C65S). The C65P substitution in the Rubisco small subunit causes a decrease in CO₂/O₂ specificity but improves holoenzyme thermal stability. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.

Species:

Chlamydomonas

Locus:

RBCS2