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MUT-4859 rbcL-L290F/rbcS1-WT mt+
Datasheet
Catalog No: ALS-03994
Product Details
Subategory:
Mutants
Description:
This strain was created transforming rbcL-L290F/rbcSΔ-CAL005.01.13 mt+ with the pSS1 plasmid that contains the Rubisco rbcS1 small-subunit gene. Like the original rbcL-L290F mutant, it can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The mutant was created for comparison with rbcL-L290F/rbcS1-L66G mt+. It has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Species:
Chlamydomonas
Locus:
rbcL, RBCS1
Chromosome:
Chloroplast,2
Phenotype:
Requires acetate at 35 °C; temperature-conditional
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For Research Use Only. Not For Clinical Use.