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Description:

MUT-4861 rbcL-L290F/rbcS2-N54S mt+ (L290F/N54S/ΔSS1)

Catalog No: ALS-03996

Product Details

Subategory:
Mutants
Description:
Plasmid pSS2-N54S was used to transform rbcL-L290F/rbcSΔ-CAL005.01.13 mt+. This strain is like revertant R116-1B, but it lacks the wild-type rbcS1 gene. The small-subunit N54S substitution increases the CO2/O2 specificity of the original large-subunit L290F mutant enzyme. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL, RBCS2
Chromosome:
Chloroplast,2

For Research Use Only. Not For Clinical Use.