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MUT-4863 rbcL-L290F/rbcS2-C65S mt+ (L290F/C65S/ΔSS1)
Datasheet
Catalog No: ALS-03998
Product Details
Subategory:
Mutants
Description:
Plasmid pNESS2-C65S was used to transform rbcL-L290F/rbcSΔ-CAL005.01.13 mt+. This strain is like revertant R88-5A, but it lacks the wild-type rbcS1 gene. The small-subunit C65S substitution increases the CO2/O2 specificity of the original large-subunit L290F mutant enzyme. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL, RBCS2
Chromosome:
Chloroplast,2
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For Research Use Only. Not For Clinical Use.