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MUT-4865 rbcS1-L66G mt-
Catalog No: ALS-04000
Product Details
Subategory:
Mutants
Description:
Using standard methods of directed mutagenesis and nuclear-gene transformation of rbcSΔ-T60-3 mt- (MUT-4690), an L66G substitution (CTG-GGC) in the Rubisco small subunit was created. This mutant was created to see whether an L66G substitution in the nuclear-encoded small subunit could suppress the biochemical defects that arise from an L290F substitution in the chloroplast-encoded large subunit. The L66G substitution increases the thermal stability of wild-type Rubisco. This strain has been maintained with acetate medium in darkness since its creation.
Species:
Chlamydomonas
Locus:
RBCS1
Chromosome:
2
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For Research Use Only. Not For Clinical Use.