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MUT-4868 rbcL-K175C mt+
Datasheet
Catalog No: ALS-04003
Product Details
Subategory:
Mutants
Description:
Using plasmid p699, rbcL direct mutagenesis, chloroplast transformation of wild-type 2137 mt+ (cloned), selection for spectinomycin resistance in the dark, and screening for a homoplasmic acetate-requiring phenotype to create a K175C substitution (AAA-TGT) in the Rubisco large subunit were performed. The K175C substitution causes a 90% decrease in Rubisco holoenzyme and a 99% decrease in Rubisco carboxylase activity. This mutant was created to investigate the role of active-site Lys-175 in catalysis. It has been maintained with acetate medium in darkness since its creation.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.