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MUT-4873 rbcL-K334R mt+
Datasheet
Catalog No: ALS-04008
Product Details
Subategory:
Mutants
Description:
Using plasmid p699, rbcL direct mutagenesis, chloroplast transformation of wild-type 2137 mt+ (cloned), selection for spectinomycin resistance in the dark, and screening for a homoplasmic acetate-requiring phenotype to create a K334R substitution in the Rubisco large subunit were performed. This active-site mutant was created to see whether suppressor substitutions could be selected that would complement a deficiency in Rubisco carboxylation. It has been maintained with acetate medium in darkness since its creation.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.