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MUT-4875 rbcL-R32K mt+
Catalog No: ALS-04010
Product Details
Subategory:
Mutants
Description:
Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696), an R32K substitution was created in the Rubisco large subunit. This mutant was created to investigate phylogenetic differences in the Rubisco large subunit that may be responsible for pyrenoid formation in algae. See also rbcL-R32K/A222S/C247S. The R32K substitution does not affect Rubisco catalysis or pyrenoid formation. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.