Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696), a C247S substitution was created in the Rubisco large subunit. This mutant was created to investigate phylogenetic differences in the Rubisco large subunit that may be responsible for pyrenoid formation in algae. See also rbcL-R32K/A222S/C247S. The C247S substitution does not affect Rubisco catalysis or pyrenoid formation. Cys-247 forms a disulfide bond with Cys-247 in a neighboring large subunit, which may be the only disulfide bond in the Rubisco holoenzyme. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.

Species:

Chlamydomonas

Locus:

rbcL

Chromosome:

Chloroplast