Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696), three substitutions (R32K, A222S, and C247S) were created together in the Rubisco large subunit. This mutant was created to investigate phylogenetic differences in the Rubisco large subunit that may be responsible for pyrenoid formation in algae. See also rbcL-R32K, rbcL-A222S, and rbcL-C247S. The three combined substitutions do not affect Rubisco catalysis or pyrenoid formation. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.

Species:

Chlamydomonas

Locus:

rbcL

Chromosome:

Chloroplast