Subategory:

Mutants

Description:

Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 11-6F grows slowly on minimal medium in the light and displayed a mendelian pattern of inheritance. It has PSI-deficient fluorescence-induction kinetics, but only a 50% decrease in CO₂ fixation. This is the original isolate (see also MUT-1229 11-6F mt-). It has been maintained with acetate medium in darkness to prevent selection for revertants or suppressors.

Species:

Chlamydomonas

Phenotype:

Requires acetate; leaky