Subategory:

Mutants

Description:

Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 10-2E displayed uniparental inheritance, and has normal fluorescence-induction kinetics. Recombination tests showed that 10-2E is at the same locus (psp-u-1) as 8-31, 8-35, 10-7C, and 12-10B indicating that it may lack photosynthetic phosphorylation due to an atp-gene mutation. A different photosynthetic phosphorylation locus (psp-u-2) was defined by mutant 12-5A (MUT-2260). This strain was recovered from a cross between the original 10-2E mt+ and pf2 mt-, and has been maintained with acetate medium in darkness to prevent selection for revertants and non-light-sensitive suppressors.

Species:

Chlamydomonas

Chromosome:

Chloroplast

Phenotype:

Requires acetate; sensitive to light