Subategory:

Mutants

Description:

Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 21gr mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 8-36C displayed uniparental inheritance, and lacks photosystem II activity. Recombination tests showed that 8-36C is at the same locus (pst-u-1) as 10-4, 11-1A, 11-3D, 11-4D, and 12-3C. Because the 8-36C mutation was shown to be linked with a mutation conferring herbicide resistance, subsequent studies confirmed that 8-36C results from a deletion in the psbA gene similar to that of FUD7 (MUT-4147). This strain was recovered from a cross between the original 8-36C mt+ and pf2 mt-, and has been maintained with acetate medium in darkness to prevent selection for revertants and non-light-sensitive suppressors.

Species:

Chlamydomonas

Locus:

psbA

Chromosome:

Chloroplast

Phenotype:

Requires acetate; sensitive to light