Subategory:

Mutants

Description:

Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 11-3D displayed uniparental inheritance, and lacks photosystem II activity. Recombination tests showed that 11-3D is at the same locus (pst-u-1) as 8-36C, 10-4, 11-1A, 11-4D, and 12-3C. Because 8-36C, 11-1A, and 11-4D result from deletions in psbA, 11-3D may also result from a psbA mutation. This strain was recovered from a cross between the original 11-3D mt+ (MUT-2052) and pf2 mt-, and has been maintained with acetate medium in darkness to prevent selection for revertants and non-light-sensitive suppressors.

Species:

Chlamydomonas

Chromosome:

Chloroplast

Phenotype:

Requires acetate; sensitive to light