Subategory:

Mutants

Description:

Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 11-4D displayed uniparental inheritance, and lacks photosystem II activity. Recombination tests showed that 11-4D is at the same locus (pst-u-1) as 8-36C, 10-4, 11-1A, 11-3D, and 12-3C. Mutants 8-36C, 11-1A, and 11-4D were shown to result from deletions in psbA. However, the extent of the deletions in 11-1A and 11-4D differs from that in 8-36C and FUD7 (MUT-4147). This strain was recovered from a cross between the original 11-4D mt+ and pf2 mt-, and has been maintained with acetate medium in darkness to prevent selection for revertants and non-light-sensitive suppressors.

Species:

Chlamydomonas

Locus:

psbA

Chromosome:

Chloroplast

Phenotype:

Requires acetate; sensitive to light