Subategory:

Mutants

Description:

Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 10-3C displayed uniparental inheritance, and PSI-deficient fluorescence-induction kinetics. Recombination tests showed that 10-3C is at the same locus (pso-u-1) as 12-7. This PSI-deficient mutant could grow photoautotrophically, but the strain they used (MUT-2046) likely reverted and has since been lost from the culture collection. This strain was recovered from a cross between the original 10-3C mt+ and pf2 mt-, and has been maintained with acetate medium in darkness to prevent selection for revertants and non-light-sensitive suppressors.

Species:

Chlamydomonas

Chromosome:

Chloroplast

Phenotype:

Requires acetate; sensitive to light