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MUT-4904 12-7 mt+ (pso-u-1-12-7)
Datasheet
Catalog No: ALS-04039
Product Details
Subategory:
Mutants
Description:
Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 12-7 displayed uniparental inheritance, and PSI-deficient fluorescence-induction kinetics. Recombination tests showed that 12-7 is at the same locus (pso-u-1) as 10-3C. This strain was recovered from a cross between the original 12-7 mt+ and pf2 mt-, and has been maintained with acetate medium in darkness to prevent selection for revertants and non-light-sensitive suppressors.
Species:
Chlamydomonas
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.