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Description:

MUT-4905 15-1L mt+

Catalog No: ALS-04040

Product Details

Subategory:
Mutants
Description:
Using established methods of 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 15-1L displayed uniparental inheritance, and has an 80% decrease in Rubisco carboxylase activity and a 95% decrease in whole-chain electron transport. These pleiotropic alterations may arise from a defect in chloroplast protein synthesis. This strain has been maintained with acetate medium in darkness to prevent selection for revertants and non-light-sensitive suppressors.
Species:
Chlamydomonas
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light

For Research Use Only. Not For Clinical Use.