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MUT-4924 rbcLΔ/rbcS1-LSRR mt-
Datasheet
Catalog No: ALS-04059
Product Details
Subategory:
Mutants
Description:
This mutant was created by nuclear transformation of rbcLΔ/rbcSΔ-1A mt- (MUT-4697) with a plasmid (pSS1-LSRR) that has the Chlamydomonas Rubisco small-subunit coding region of pSS1-ITP replaced with the codon-optimized sequence of Rhodospirillum rubrum Rubisco. The mutant grows very slowly on minimal medium only when provided with 5% CO2 in air. It expresses only a low level of R. rubrum Rubisco. The strain has been maintained with acetate medium in darkness since its recovery.
Species:
Chlamydomonas
Locus:
rbcL, RBCS1
Chromosome:
Chloroplast,2
Phenotype:
Requires elevated CO2 for photosynthetic growth; zeocin and spectinomycin resistant
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For Research Use Only. Not For Clinical Use.