Products
Online Inquiry
MUT-4925 rbcLΔ/rbcS1-10SS-LSRR mt-
Datasheet
Catalog No: ALS-04060
Product Details
Subategory:
Mutants
Description:
This mutant was created by nuclear transformation of rbcLΔ/rbcSΔ-1A mt- (MUT-4697) with a plasmid (pSS1-10SS-LSRR) that has the Chlamydomonas Rubisco small-subunit coding region of pSS1-ITP after residue 10 replaced with the codon-optimized sequence of Rhodospirillum rubrum Rubisco. The mutant grows slowly on minimal medium only when provided with 5% CO2 in air. It expresses only a low level of R. rubrum Rubisco, but more than mutant rbcLΔ/rbcS1-LSRR mt-. The strain has been maintained with acetate medium in darkness since its recovery.
Species:
Chlamydomonas
Locus:
rbcL, RBCS1
Chromosome:
Chloroplast,2
Phenotype:
Requires elevated CO2 for photosynthetic growth; zeocin and spectinomycin resistant
Related Products
For Research Use Only. Not For Clinical Use.