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MUT-4926 rbcS1-10SS-LSRR mt-
Datasheet
Catalog No: ALS-04061
Product Details
Subategory:
Mutants
Description:
This mutant was created by nuclear transformation of rbcSΔ-T60-3 mt- (MUT-4690) with a plasmid (pSS1-10SS-LSRR) that has the Chlamydomonas Rubisco small-subunit coding region of pSS1-ITP after residue 10 replaced with the codon-optimized sequence of Rhodospirillum rubrum Rubisco. The mutant grows slowly on minimal medium only when provided with 5% CO2 in air. It expresses only a low level of R. rubrum Rubisco, but more than mutant rbcLΔ/rbcS1-LSRR mt-. The strain has been maintained with acetate medium in darkness since its recovery.
Species:
Chlamydomonas
Locus:
RBCS1
Chromosome:
2
Phenotype:
Requires elevated CO2 for photosynthetic growth
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For Research Use Only. Not For Clinical Use.