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MUT-4927 rbcLΔ/rbcS1-SSAT pf2 mt+
Datasheet
Catalog No: ALS-04062
Product Details
Subategory:
Mutants
Description:
This Rubisco rbcL knock-out mutant was created by replacing chloroplast rbcL of rbcS1-SSAT pf2 mt+ (MUT-4764) with aadA. Whereas the original SSAT strain expresses functional Rubisco comprised of Chlamydomonas large subunits and Arabidopsis small subunits, this mutant can express only Arabidopsis rbcS. The mutant was created to see whether functional Arabidopsis Rubisco could be expressed in Chlamydomonas. It has been cloned to homoplasmicity, and maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL, RBCS1, PF2
Chromosome:
Chloroplast,2,11
Phenotype:
Requires acetate; sensitive to light; spectinomycin resistant
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For Research Use Only. Not For Clinical Use.