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MUT-4928 rbcLΔ/rbcS1-SSSO pf2 mt+
Datasheet
Catalog No: ALS-04063
Product Details
Subategory:
Mutants
Description:
This Rubisco rbcL knock-out mutant was created by replacing chloroplast rbcL of rbcS1-SSSO pf2 mt+ (MUT-4762) with aadA. Whereas the original SSSO strain expresses functional Rubisco comprised of Chlamydomonas large subunits and spinach small subunits, this mutant can express only spinach rbcS. The mutant was created to see whether functional spinach Rubisco could be expressed in Chlamydomonas. It has been cloned to homoplasmicity, and maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL, RBCS1, PF2
Chromosome:
Chloroplast,2,11
Phenotype:
Requires acetate; sensitive to light; spectinomycin resistant
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For Research Use Only. Not For Clinical Use.