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MUT-4929 rbcLΔ/rbcS1-SSHA pf2 mt+
Datasheet
Catalog No: ALS-04064
Product Details
Subategory:
Mutants
Description:
This Rubisco rbcL knock-out mutant was created by replacing chloroplast rbcL of rbcS1-SSHA pf2 mt+ (MUT-4766) with aadA. Whereas the original SSHA strain expresses functional Rubisco comprised of Chlamydomonas large subunits and sunflower small subunits, this mutant can express only sunflower rbcS. The mutant was created to see whether functional sunflower Rubisco could be expressed in Chlamydomonas. It has been cloned to homoplasmicity, and maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL, RBCS1, PF2
Chromosome:
Chloroplast,2,11
Phenotype:
Requires acetate; sensitive to light; spectinomycin resistant
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For Research Use Only. Not For Clinical Use.