Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696), a C369A substitution (TGT-GCC) was created in the Rubisco large subunit. Because Cys-369 is methylated, this mutant was created to investigate the role of the modified residue in Rubisco structure or function. The C369A substitution causes a decrease in Rubisco CO₂/O₂ specificity, but does not affect the photosynthetic growth of the mutant cells. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.

Species:

Chlamydomonas

Locus:

rbcL

Chromosome:

Chloroplast