Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696), a C369V substitution (TGT-GTT) was created in the Rubisco large subunit. Because Cys-369 is methylated in Chlamydomonas Rubisco, but replaced by Val in plant Rubisco, the mutant was created to investigate the role of the modified residue in Rubisco structure or function. The C369F substitution causes small increases in Rubisco CO₂/O₂ specificity and carboxylation catalytic efficiency. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may alter Rubisco function.

Species:

Chlamydomonas

Locus:

rbcL

Chromosome:

Chloroplast