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MUT-5018 rbcL-V149Q/I282H mt+ (G149-282)
Datasheet
Catalog No: ALS-04095
Product Details
Subategory:
Mutants
Description:
Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696), two substitutions (V149Q and I282H) together in the Rubisco large subunit were created. This mutant (also named G149-282) represents one of 15 "groups" of amino acids that differ between Chlamydomonas and land plants. It was created to investigate phylogenetic differences that influence Rubisco catalysis. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.