Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696), four substitutions (G442N, D443E, V444I, and S447E) together in the Rubisco large subunit were created. This mutant (also named G442-447) represents one of 15 "groups" of amino acids that differ between Chlamydomonas and land plants. It was created to investigate phylogenetic differences that influence Rubisco catalysis. The mutant has small decreases in photosynthetic growth and the amount of Rubisco holoenzyme. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.

Species:

Chlamydomonas

Locus:

rbcL

Chromosome:

Chloroplast