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MUT-5031 rbcLΔ/rbcS1-ABSO mt+
Datasheet
Catalog No: ALS-04108
Product Details
Subategory:
Mutants
Description:
This Rubisco rbcL knock-out mutant was created by using aadA to replace chloroplast rbcL of rbcL-Penta/rbcS1-ABSO mt+ (MUT-4848). The mutant lacks Rubisco large subunits, but is capable of expressing the ABSO small subunit, which was previously engineered by replacing the Chlamydomonas betaA-betaB loop with the loop from the small subunit of spinach Rubisco. The mutant was created to investigate large-subunit amino acids that differ between Chlamydomonas and land plants, and that interact with small-subunit amino acids. This strain has been cloned to homoplasmicity, and maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL, RBCS1
Chromosome:
Chloroplast,2
Phenotype:
Requires acetate; sensitive to light; spectinomycin resistant
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For Research Use Only. Not For Clinical Use.