Subategory:

Mutants

Description:

Using plasmid p699, rbcL direct mutagenesis, chloroplast transformation of wild-type 2137 mt+ (MUT-4887), selection for spectinomycin resistance in the dark, and screening for a homoplasmic acetate-requiring phenotype to create 34 substitutions together in the Rubisco large subunit (A11V, V30E, V31T, R32K, M42V, C53A, D86H, I105L, V149Q, G168P, V221C, V235I, C256F, K258R, I265V, I282H, R305K, L326I, V341I, M349L, C369V, M375L, V391T, A398S, C399V, T428V, G442N, D443E, V444I, S447E, D470E, T471A, I472M, and K474T) were performed. The mutant lacks Rubisco holoenzyme. It was created to investigate phylogenetic differences that influence Rubisco catalysis. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or suppress light sensitivity.

Species:

Chlamydomonas

Locus:

rbcL

Chromosome:

Chloroplast

Phenotype:

Requires acetate; sensitive to light