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MUT-5038 rbcL-V221C/C256F/K258R mt+
Datasheet
Catalog No: ALS-04115
Product Details
Subategory:
Mutants
Description:
Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ-MX3312 mt+ (MUT-4696), three substitutions (V221C, C256F, and K258R) together in the Rubisco large subunit were created. This mutant was created to investigate the molecular basis for the increased CO2/O2 specificity of mutant rbcL-Penta/rbcS1-ABSO mt+ (MUT-4848). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.