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Description:

MUT-5040 rbcL-C256F/K258R/rbcS1-ABSO mt+

Catalog No: ALS-04117

Product Details

Subategory:
Mutants
Description:
Using standard methods of directed mutagenesis and chloroplast transformation of rbcLΔ/rbcS1-ABSO mt+, two substitutions (C256F and K258R) together in the Rubisco large subunit were created. This mutant was created to investigate the molecular basis for the increased CO2/O2 specificity of mutant rbcL-Penta/rbcS1-ABSO mt+ (MUT-4848). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL, RBCS1
Chromosome:
Chloroplast,2

For Research Use Only. Not For Clinical Use.