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MUT-5595 amc5; NUOB10(C91S)-FLAG mt+ [transformant G]
Catalog No: ALS-04595
Product Details
Subategory:
Mutants
Description:
Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10C91S-FLAG; APHVII. This is the amc5 strain complemented with the NUOB10-FLAG gene encoding C91S substitution. Mutagenesis of amc5 with cosmid plasmid pRS426 containing the NUOB10 gene encoding the C91S substitution with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant. This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.
Species:
Chlamydomonas
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For Research Use Only. Not For Clinical Use.